Gene number
836
Conjugated
PURE
Species reactivity
Human
Molecular weight
17 kDa
UniProt antigen number
P42574
French translation
anticorps
Alternative names
CASP3, CPP32
Clone
Rabbit Polyclonal
Tested application
Western Blot (1:3000)
Latin name
Oryctolagus cuniculus
Group
Polyclonals and antibodies
Regulation status
This antibody is intended for Research Use Only.
Storage buffer
PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
Technical datasheet
Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS
Shipping conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Immunogen
synthetic peptide (KLH-conjugated) corresponding to amino terminal residues adjacent to Asp175 of human caspase-3.
Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Long description
The Rabbit Polyclonal Antibody Anti Caspase-3 active is recommended to detect specifically in Western Blot technique caspase 3 (CPP-32, Apoptain, Yama, SCA-1)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Immunostep. Rabbit antibodies are very stable and can be stored for several days at room temperature. Immunostep adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
