Gene ID number
836
Modification(s)
None
French translation
anticorps
Concentration
1ug per 1ul
Conjugation
Unconjugated
Cross reactivity
Human, Mouse, Rat
Category
Primary Antibodies
Modification site(s)
Unmodified antibody
Clonality
Polyclonal antibody
Clone number
Polyclonal antibody
Tested Applications
WB, IHC-P, IF(IHC-P)
Target Antigen
caspase-3 p12 subunit
Long name
caspase-3 p12 subunit Antibody
Also known as
caspase-3 p12 subunit Antibody
Purification method
This antibody was purified via Protein A.
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Specificity
This is a highly specific antibody against caspase-3 p12 subunit.
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit
Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Synonym names
Caspase-3 subunit p12; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
Background of the target antigen
The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
