Immunoglobulin
IgG
NCBI GENE nr
836
Works with
Human
GENE symbol
CASP3
Ab raised in
Rabbit
Protein nr
P42574
KDa
30 kDa
Quantity per ml
1 mg/mL
Omim nr
14790119
Ncbi nr
NP_004337
French translation
anticorps
Type
Polyclonal
Vial with antibody
in solution
Source
homo sapiens
Antibodie's label
unlabelled serum
Immuno application
ELISA, immuno-histo-chemistry
Antigene name
caspase 3, apoptosis-related cysteine peptidase
Spicific to
At least two isoforms of Caspase-3 are known to exist.
Suspention, pH, azide
Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.
Purity
Caspase-3 Antibody is affinity chromatography purified via peptide column.
Gene names
Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3
Antibody advice
Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 ug/mL.
Antigen
Caspase-3 antibody was made against a 17 amino acid synthetic peptide near the center of human Caspase-3.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Keep at
Caspase-3 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Gene info
Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.
