Purification method
N/A
Concentration
N/A
Immunoglobulin isotype
IgG2a
Also known as
Caspase-3
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Tested applications:
Western Blot
Clone
SB71b1 (5G5A11)
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Form/Appearance
Purified (UNLB) Antibody
Other gene names
CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1
Gene name synonims
CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1
Gene name
CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.1mg purified immunoglobulin in 0.1 mL of 100 mM borate buffered saline, pH 8.0; Store the antibody ats should be kept in the range of 1-7 degrees Celsius..
Other names
caspase-3 preproprotein; Caspase-3; caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
Reacts with human Caspase 31The Caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase 3 (CPP32, Yama, apopain) is one of the best characterized caspases in terms of specificity and function in apoptosis. It is a ubiquitously expressed protein in mammals and is highly prevalent in cells with lymphocytic origins, indicating a regulatory role in the immune system. Caspase 3 plays a critical role during embryonic development by inducing morphogenetic cell death in the mammalian brain and has also been linked to chromatin condensation, DNA fragmentation and blebbing. The SB71b monoclonal antibody detects a band at ~32 and 17 kD, corresponding to the full length and cleaved forms of Caspase 3.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
