Gene ID number 836
Modification site None
Additional conjugation Cy5.5
Swiss Prot P42574
Target Antigen Caspase-3
Subcellular location Cytoplasm
French translation anticorps
Concentration 1ug per 1ul
Excitation Emission 625,650nm/670nm
Clone Polyclonal antibody
Tested applications FCM, IF(IHC-P), IF(ICC)
Category Conjugated Primary Antibodies
Host Organism Rabbit (Oryctolagus cuniculus)
Crossreactivity Human, Mouse, Rat, Bovine, Goat
Purification method This antibody was purified via Protein A.
Long name Caspase 3 Polyclonal Antibody, Cy5 Conjugated
Recommended dilutions FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Specificity This is a highly specific antibody against Caspase-3.
Source KLH conjugated synthetic peptide derived from human Caspase 3(Active)
Crossreactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Synonyms CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3
Storage conditions Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Description Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Antigen background Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.