Gene ID number
836
Conjugation
FITC
Modification site
None
Swiss Prot
P42574
Target Antigen
Caspase-3
Subcellular location
Cytoplasm
French translation
anticorps
Immunogen_range
60-100/277
Modification
Unmodified
Clonality
Polyclonal
Excitation Emission
494nm/518nm
Concentration
1ug per 1ul
Additional conjugation
Anti-FITC Antibody
Clone
Polyclonal antibody
Tested applications
FCM, IF(IHC-P), IF(ICC)
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Crossreactivity
Human, Mouse, Rat, Bovine, Goat
Purification method
This antibody was purified via Protein A.
Long name
Caspase 3 Polyclonal Antibody, FITC Conjugated
Recommended dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Specificity
This is a highly specific antibody against Caspase-3.
Source
KLH conjugated synthetic peptide derived from human Caspase 3(Active)
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Synonyms
CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Antigen background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
