• 024140089086
  • de@gentaur.com
Caspase 3 Antibody, FITC Conjugated

Size

Caspase 3 Antibody, FITC Conjugated

0.1ml

Catalog no.

bs-0081R-FITC

Price

333 EUR

Buy Caspase 3 Antibody, FITC Conjugated at gentaur.com

Gene ID number

836

Conjugation

FITC

Modification site

None

Swiss Prot

P42574

Target Antigen

Caspase-3

Subcellular location

Cytoplasm

French translation

anticorps

Immunogen_range

60-100/277

Modification

Unmodified

Clonality

Polyclonal

Excitation Emission

494nm/518nm

Concentration

1ug per 1ul

Additional conjugation

Anti-FITC Antibody

Clone

Polyclonal antibody

Tested applications

FCM, IF(IHC-P), IF(ICC)

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Crossreactivity

Human, Mouse, Rat, Bovine, Goat

Purification method

This antibody was purified via Protein A.

Long name

Caspase 3 Polyclonal Antibody, FITC Conjugated

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Specificity

This is a highly specific antibody against Caspase-3.

Source

KLH conjugated synthetic peptide derived from human Caspase 3(Active)

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Synonyms

CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Antigen background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.