Gene ID number
836
Conjugation
Cy3
Modification site
None
Swiss Prot
P42574
Target Antigen
Caspase-3
Subcellular location
Cytoplasm
French translation
anticorps
Immunogen_range
60-100/277
Modification
Unmodified
Clonality
Polyclonal
Concentration
1ug per 1ul
Additional conjugation
cy3 conjugation kit
Excitation Emission
512,550nm/570,615nm
Clone
Polyclonal antibody
Tested applications
FCM, IF(IHC-P), IF(ICC)
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Crossreactivity
Human, Mouse, Rat, Bovine, Goat
Purification method
This antibody was purified via Protein A.
Long name
Caspase 3 Polyclonal Antibody, Cy3 Conjugated
Recommended dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Specificity
This is a highly specific antibody against Caspase-3.
Source
KLH conjugated synthetic peptide derived from human Caspase 3(Active)
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Synonyms
CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Caspase-3 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
Antigen background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
