Usage
NA
Reactivity
Human
Swiss-Prot
P42574
BiowMW
~ 17 kDa
French translation
anticorps
Applications
WB ICC/IF IHC
Latin name
Oryctolagus cuniculus
Immunogen
Recombinant antibody.
Purification&Purity
Protein A affinity purified
Application_all
WB: 1:1000-1:2000 ICC/IF: 1:50-1:200 IHC: 1:50-1:200
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Specificity
This antibody detects endogenous levels of Actived-Caspase-3 p17 protein.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name
Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
