Swiss-Prot
P42574
BiowMW
31 kDa
French translation
anticorps
Reactivity
Human, Mouse
Applications
WB ICC IHC FC
Purification&Purity
Peptide affinity purified
Group
Polyclonals and antibodies
Application_all
WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200
Note
For research use only, not for use in diagnostic procedure.
Immunogen
Synthetic peptide (KLH-coupled) witihin human Caspase-3 N-terminal.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Specificity
Caspase-3 polyclonal antibody detects endogenous levels of Caspase-3 protein.
Storage&Stability
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Usage
Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution. Positivecontrol: Lane 1: Human lung Lane 2: Human brain Lane 3: Jurkat Lane 4: F9 Lane 5: NCCTI Lane 6: Daudi Lane 7: Raji Lane 8: MCF-7 Lane 9: Hela
Alternative Name
CASP-3, CPP-32, SCA-1, A830040C14Rik, Apopain, CASP-3, CASP3, CASP3, Casp3a, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine protease CPP32, EC 3.4.22.56, LICE, mldy, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, PARP cleavage protease, Procaspase3, Protein Yama, SCA 1, SCA-1, SREBP cleavage activity 1, Yama,
