Applications
WB
Swiss-Prot
P42574
BiowMW
~ 17 kDa
French translation
anticorps
Reactivity
Human,Mouse,Rat
Application_all
WB: 1:500~1:1000
Group
Polyclonals and antibodies
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.
Alternative Name
Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Usage
Western blot (WB) analysis of Caspase-3 p17 (D175) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with Lps Lane2:Raw264.7 cell lysate treated with Lps Lane3:PC12 cell lysate treated with Lps
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Specificity
Caspase-3 p17 (D175) polyclonal antibody detects endogenous levels of the large fragment (17 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.
