Applications WB IHC
French translation anticorps
BiowMW ~ 17, 35 kDa
Group Polyclonals and antibodies
Application_all WB: 1:500~1:1000 IHC: 1:50~1:200
Note For research use only, not for use in diagnostic procedure.
Product Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity Actived-Caspase-3 p17 polyclonal antibody detects endogenous levels of Actived-Caspase-3 p17 protein.
Immunogen Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.
Alternative Name Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
Properties If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification&Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE).
Description Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Usage Western blot (WB) analysis of Actived-Caspase-3 p17 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)