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Actived-Caspase-3 p17 polyclonal antibody

Size

Actived-Caspase-3 p17 polyclonal antibody

50ul

Catalog no.

BS7004-50ul

Price

318 EUR

Buy Actived-Caspase-3 p17 polyclonal antibody at gentaur.com

Swiss-Prot

P42574

Applications

WB IHC

French translation

anticorps

BiowMW

~ 17, 35 kDa

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity

Actived-Caspase-3 p17 polyclonal antibody detects endogenous levels of Actived-Caspase-3 p17 protein.

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.

Alternative Name

Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE).

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of Actived-Caspase-3 p17 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)