Tested applications
WB
Uniprot #
P42574
Category
Antibody
Recognised antigen
Caspase-3
French translation
anticorps
Conjugation
Unconjugated
Purity
Antigen affinity
Clone
Polyclonal antibody
Recommended dilutions
Western blot: 0.5-1ug/ml
Form
Antigen affinity purified
Clonality
Polyclonal (rabbit origin)
Host animal
Rabbit (Oryctolagus cuniculus)
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Intented use
This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Immunogen
An amino acid sequence from the N-terminus of human Caspase-3 (DAANLRETFRNLKYEVRNKND) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the large subunit.
Notes
The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody p17 may be required due to differences in protocols and secondary/substrate sensitivity.
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Storage
After reconstitution, the Caspase-3 antibody p17 can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Description
CASP3(Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease(caspase) family. Tiso et al.(1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.
