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Caspase-3 Antibody

Size

Caspase-3 Antibody

0.1mg

Catalog no.

R30359

Price

389 EUR

Buy Caspase-3 Antibody at gentaur.com

Uniprot #

P42574

Category

Antibody

Tested applications

WB, IHC-P

Recognised antigen

Caspase-3

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Immunogen

An amino acid sequence from the C-terminus of mouse Caspase-3 (CSMLKLYAHKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.

Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Additional description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Storage

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

Caspase-3 is a protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.