WB, FACS, IHC, ELISA
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
In 1X PBS, pH 7.4, with 0.09% sodium azide
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50
This anti-Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..
A portion of amino acids 60-90 from the human protein was used as the immunogen for this anti-Caspase-3 antibody.
Titration of the anti-Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Aliquot the anti-Caspase-3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.