Verified reactivity
Human
Verified applications
ELISA
Protein number
P42574
Research main area
Biology
Use for
6 months
Shipping requirements
Blue ice
Assay duration
1-5 hours
Sensitivity limit
0,078 ng/ml
ELISA detection
Colorimetric
Assay class
Please contact us
Estimated production time
7-11 business days
Detection limits
0.312 ng/ml-20 ng/ml
Gene number
Please refer to GenBank
Alternate protein number
Please refer to SwissProt
Samples to be analyzed
serum, plasma, cell lysates, tissue homogenates
Gene name
caspase 3, apoptosis-related cysteine peptidase
Assay principle
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Please see ELISA's datasheet, otherwise contact us
ELISA's stability
Please see ELISA's datasheet, otherwise contact us
Protocol
Please see ELISA's datasheet, otherwise contact us
Notes
For research use only. Not for diagnostic procedures.
QC
The kit is manufactured at ISO 9001 certified facilities.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
ELISA's specificity
To know the specificity of this test please feel free to contact us
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Alternate gene name
CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
