Storage condition
4°C
Kit's other name
none
Species reactivity
Human
Maximum time can be stored
6 months
Shipping condition
gel pack
Category
ELISA kit
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Samples tested
Human serum, plasma, tissue homogenates and other biological fluids.
Applications
Quantitative measurement of Caspase-3 in human serum, plasma, tissue homogenates and other biological fluids.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of Caspase:3 in human serum, plasma, tissue homogenates and other biological fluids.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Kit's benefits
Easy, convenient and time-saving method to measure the level of Caspase-3 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.313-20 ng/ml Sensitivity: < 0.188 ng/ml
Contents
• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Kit's description
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. BioVision’s Caspase-3 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human Caspase-3 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Caspase-3 captured from the samples.
