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Caspase-3 Colorimetric Assay Kit

Size

Caspase-3 Colorimetric Assay Kit

400 assays

Catalog no.

K106-400

Price

955 EUR

Buy Caspase-3 Colorimetric Assay Kit at gentaur.com

Kit's other name

none

Storage condition

-20°C

Shipping condition

gel pack

Species reactivity

Mammalian

Maximum time can be stored

12 months

Category

Apoptosis Assay Kit

Samples tested

Cell and tissue lysates

Test

Biovision supplies other types of Assays as 1.

Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Contents

• Cell Lysis buffer • 2X Reaction Buffer • DEVD-pNA (4 mM) • DTT (1 M) • Dilution Buffer

Kit's benefits

• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Highlights

• Detection method: Absorbance (400 or 405 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit's description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.