Kit's other name
none
Storage condition
-20°C
Shipping condition
gel pack
Species reactivity
Mammalian
Maximum time can be stored
12 months
Category
Apoptosis Assay Kit
Samples tested
Cell and tissue lysates
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Contents
• Cell Lysis buffer • 2X Reaction Buffer • DEVD-pNA (4 mM) • DTT (1 M) • Dilution Buffer
Kit's benefits
• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Highlights
• Detection method: Absorbance (400 or 405 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit's description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
