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Caspase-3 Fluorometric Assay Kit

Size

Caspase-3 Fluorometric Assay Kit

400 assays

Catalog no.

K105-400

Price

955 EUR

Buy Caspase-3 Fluorometric Assay Kit at gentaur.com

Kit's other name

none

Storage condition

-20°C

Shipping condition

gel pack

Species reactivity

Mammalian

Maximum time can be stored

12 months

Category

Apoptosis Assay Kit

Samples tested

Cell and tissue lysates

Test

Biovision supplies other types of Assays as 1.

Contents

• Cell Lysis Buffer • 2X Reaction Buffer • DEVD-AFC (1 mM) • DTT (1 M)

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Kit's benefits

• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

Highlights

• Detection method: fluorecence (400 nm excitation filter and 505 nm emission filter) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit's description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.