Kit's other name none
Storage condition -20°C
Shipping condition gel pack
Species reactivity Mammalian
Maximum time can be stored 12 months
Category Apoptosis Assay Kit
Samples tested Cell and tissue lysates
Test Biovision supplies other types of Assays as 1.
Contents • Cell Lysis Buffer • 2X Reaction Buffer • DEVD-AFC (1 mM) • DTT (1 M)
Applications Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Description Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Kit's benefits • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity
Highlights • Detection method: fluorecence (400 nm excitation filter and 505 nm emission filter) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit's description Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.