Is this a salt?
No
Is it cell-permeable?
No
Reconstitute instructions
N/A
CAS number
N/A
Tissue
cell
Storage condition
-20°C
Molecular weight
638.58
Physical appearance
Liquid
Shipping condition
gel pack
Maximum time for storage
6-12 months
Purification
>98% by HPLC
Supplied with
In DMSO ( 4 mM)
Category
Caspase Substrate
Peptide sequence
Ac-Asp-Glu-Val-Asp-pNA
Molecular formula
C₂₆H₃₄N₆O₁₃
Storage instructions
Protect from light and moisture
Other name
Ac-DEVD-pNA, Caspase-3 Substrate, Colorimetric
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.
Description
Ready-to-use colorimetric substrate for CPP32/caspase-3 and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate DEVD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amounts of caspase assays.
