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Caspase-3 Substrate DEVD-AFC for cell activation

Size

Caspase-3 Substrate DEVD-AFC for cell activation

200 assays

Catalog no.

1007-200

Price

327 EUR

Buy Caspase-3 Substrate DEVD-AFC for cell activation at gentaur.com

Is this a salt?

No

Is it cell-permeable?

No

Reconstitute instructions

N/A

Molecular weight

729

CAS number

N/A

Tissue

cell

Storage condition

-20°C

Physical appearance

Liquid

Shipping condition

gel pack

Maximum time for storage

6-12 months

Purification

>98% by HPLC

Supplied with

In DMSO (1 mM)

Category

Caspase Substrate

Peptide sequence

Ac-Asp-Glu-Val-Asp-AFC

Molecular formula

C₃₀H₃₃F₃N₅O₁₃

Storage instructions

Potect from light and moisture

Other name

Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic

Additional description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.

Description

Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.