Is this a salt?
No
Is it cell-permeable?
No
Reconstitute instructions
N/A
Molecular weight
729
CAS number
N/A
Tissue
cell
Storage condition
-20°C
Physical appearance
Liquid
Shipping condition
gel pack
Maximum time for storage
6-12 months
Purification
>98% by HPLC
Supplied with
In DMSO (1 mM)
Category
Caspase Substrate
Peptide sequence
Ac-Asp-Glu-Val-Asp-AFC
Molecular formula
C₃₀H₃₃F₃N₅O₁₃
Storage instructions
Potect from light and moisture
Other name
Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.
Description
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
