Clone:
RM250
This antibody reacts with:
Human
Antibody host:
Rabbit
Form of antibody:
Liquid
This antibody has been succesfully tested for use in following methods:
IHC, WB
Shipping conditions:
gel pack
French translation
anticorps
Target of this antibody:
Caspase 3
Isotype:
Rabbit IgG
Clonality of antibody:
Monoclonal
Latin name
Oryctolagus cuniculus
Purification method:
Affinity purified using protein A
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
How to handle this antibody:
The antibody solution should be gently mixed before use.
Recommended usage:
IHC: 1:1000 -1:2500 dilution; WB: 1:1000 - 1:2000 dilution
Alternative names of antibody target:
CPP32, CASP3, apopain, procaspase3, CPP32B, SCA-1, CPP-32, Apopain, Yama
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Immunogene sequence:
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
Background information:
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
