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anti-Anti-Caspase-3 Rabbit Monoclonal Antibody

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anti-Anti-Caspase-3 Rabbit Monoclonal Antibody

Anti-Caspase-3 Rabbit Monoclonal Antibody

Catalog no.

A1120-50

Price

360 EUR

Buy anti-Anti-Caspase-3 Rabbit Monoclonal Antibody at gentaur.com

Clone:

RM250

This antibody reacts with:

Human

Antibody host:

Rabbit

Form of antibody:

Liquid

This antibody has been succesfully tested for use in following methods:

IHC, WB

Shipping conditions:

gel pack

French translation

anticorps

Target of this antibody:

Caspase 3

Isotype:

Rabbit IgG

Clonality of antibody:

Monoclonal

Latin name

Oryctolagus cuniculus

Purification method:

Affinity purified using protein A

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

Formulation of this antibody:

In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

How to handle this antibody:

The antibody solution should be gently mixed before use.

Recommended usage:

IHC: 1:1000 -1:2500 dilution; WB: 1:1000 - 1:2000 dilution

Alternative names of antibody target:

CPP32, CASP3, apopain, procaspase3, CPP32B, SCA-1, CPP-32, Apopain, Yama

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Immunogene sequence:

Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).

Background information:

Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.