Clone:
C33
Antibody host:
Mouse
Form of antibody:
Liquid
Shipping conditions:
gel pack
French translation
anticorps
Concentration of antibody:
0.2 mg/ml
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
This antibody has been succesfully tested for use in following methods:
Western blot
Purification method:
Affinity purified
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Recombinant human Caspase-3
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Alternative names of antibody target:
CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Formulation of this antibody:
100 µg (200 µg/ml) in PBS containing 50% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Recommended usage:
Western blotting (0.5-4 µg/ml). The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Background information:
The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
