For research use only.
Recombinant Human Caspase-3(CASP3),partial expressed in E.coli
MW: 20.6 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Uniprot ID: P42574; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 29-175aa; Sequence information: Partial; Tag: N-terminal 6xHis-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.