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Apoptosis Assay Kit
• Red-DEVD-FMK • Wash Buffer • Z-VAD-FMK
Detection of activated caspase:3 in living cells.
• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The red fluorescence label allows for direct detection of activated caspases in apoptotic cells
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Detection method: Fluorescence microscopy, plate reader (Ex/Em = 540/570 nm) and Flow cytometry • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Detection of activated caspase:3 in living cells.
Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase-3 Staining Kit provides a convenient means for detecting activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.